A. Getting Eggs
B. Egg Transferring
After the female lays her eggs in the vial, I usually transfer them into a 10 cm * 10 cm plastic case with sub cases so that the eggs can be placed one by one in each sub case. This is because larva ladybird is cannibal and readily eats other eggs after hatching. I do this like 30 to 60 minutes after egg oviposition. The case then is sealed with a plastic wrapper. I normally use Chinese brush or watercolor brush to transfer the eggs each into the case. I dip the brush in bowl of water and then rub the eggs off with the wet brush slowly and gently. Do not be harsh as you can damage the eggs. Seal the case with a plastic wrapper and make some small holes on it. From my experience, most eggs will hatch after two or three days. So, prepare the food all the time. Usually after hatching, the larva sits on or nearby the egg case and sucks up eveything left from the egg. So , you can have this time around to get the food. After an hour or 1.5 hr (depends on the condition), if the larva does not get any food, it will soon die out of hunger. After a week of feeding, the larva will increase in size as you can observe the differences. Once it gets bigger, try to transfer it once more into a bigger vial, to provide it with a space to pupate. The pupa takes about 4 - 5 days to develop into an adult depends on the species.
C. Summary of Egg Collecting and Transferring
As you can see from the picture, the first step is to obtain the eggs, then the second step is to transfer the eggs into the plastic case using Chinese brush dipped in water. Finally, transfer the larva as it gets bigger into a bigger vial to provide space to pupate. After pupating, it takes 4 - 5 days to develop into an adult.